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PRODUCT: Seahorse XFe96 Analyzer  


Seahorse XF Analyzers

Product details


  • Real-time Results- this integrated system reports metabolic rates in just minutes, without sample extraction or labeling.  Wave software controls the instrument and performs rate measurements on the fly for same day results.
  • Live-Cell Responses – detect responses to substrates, inhibitors, and other compounds in real time via the 4-port injection system and automated mixing while maintaining physiologic temperature (37oC).
  • Flexible Assay Design- the 96-well plate format accommodates many conditions in a single run and is best for dose-response studies and compound screening.
  • High Sensitivity- analyze as few as 5000 cells per well in the custom 96-well plate.  Cell number requirements vary by cell type; consult the Cell Reference Database for details.
  • Compatible with a variety of samples due to the precision-controlled heating tray, which can maintain 16-42oC (12oC-20oC above ambient conditions).
  • Measure mitochondrial function with the Seahorse XF Cell Mito Stress Test
  • Quantify ATP production by Glycolysis and OXPHOS simultaneously with the Seahorse XF real-time ATP rate assay
  • Generate a metabolic phenotype within one hour with the Seahorse XF Cell Energy Phenotype Test
  • Measure glycolytic rates in live cells with the Seahorse XF Glycolytic Rate Assay
  • Quickly determine dependency of cellular energy production on mitochondrial substrates with the Seahorse XF Mito Fuel Flex Test
  • Analyze 3D samples such as spheroids and islets with the Seahorse XFe96 Spheroid Plate
  • Easily create assay protocols and analyze data with Seahorse Wave software


Specification Detail
Number of Assay Wells


Microchamber volume 2 µL
Controller Combination Windows 10 computer and touchscreen display with full assay design, control, and analysis capability.
Software Design and analyze assay templates on Controller or Wave desktop.
Best For
  • Phenotypic Screening
  • Testing many conditions at one time
  • Dose-response studies
  • Spheroids
Key Advantages
  • Maximum experimental flexibility
  • Highest throughput
  • Validated for hypoxia
  • Validated for assay temperatures 16-42°C (12–20°C lower ambient temp required)
Plate Format 96-well microplate

All Agilent Seahorse XF Analyzers Feature:

  • Compatibility with both adherent and suspension cells as well as isolated mitochondria and non-mammalian samples.
  • Ability to perform up to 4 independent injections per well with automatic mixing
  • Automatic calculation of oxygen consumption rate (OCR) and extracellular acidification rate (ECAR).
  • Simultaneous measurement of OCR and ECAR in the same well
  • Analytical Sensitivity for small sample sizes
  • Label-free detection in real time
  • Windows-compatible desktop analysis software (Wave) for plotting, reporting, analyzing, and exporting your XF data